HPLC is a common method known for separation of complex samples. The sample gets applied to a column. HPLC columns are of different types making it difficult to identify the right one. Read on for you to know what you need to have in mind when selecting HPLC column.
It is essential you think about the separation methods when choosing an HPLC column. There are different ways to separate molecules. Some of the popular methods used are normal phase, reverse phase, ion exchange and hydrophilic interaction.
The column length is vital. You need first to decide on the column type for you to identify the right length. The length will be determined by your samples and separation mode. It is vital you make sure the column length you choose will achieve a balance between the resolution and analysis time. Don’t make a mistake of choosing a type of column that you have used in previous experiments to apply to a new experiment without considering the appropriate length.
It is important for you to consider the type and size of the column particles. In most cases HPLC columns are in spherical shape. The type and shape will be determined by the complexity of the samples. It is important to pay attention to the design of the column. Make sure you consider the accuracy of data provided by the specific brand of HPLC column you select. Research is important for you to select the best HPLC column.
Use the internet and view the different models of HPLC columns that are available. Your goal is to identify a brand with significant value and performance. Check online reviews to get more details about the various brands. Consider a brand that is known is trusted by many. Compare the various specifications for you to make the right decision. You can make your search easier by identifying the best brands.
Another factor to consider when choosing HPLC column is price. The rates vary based on the brand. The price is determined by the quality of the products and the specifications. Narrow your search by comparing HPLC columns that you can afford. You can request different manufacturers to provide you with what for you to make comparisons. You will be able to create a reasonable budget. Avoid HPLC columns that come at a very low rate. There is a high chance you will not get accurate results.
Consider training and maintenance of the HPLC system before making your purchase. It is important for you to begin by identifying your needs and applications for you. The hands-on experience you have will determine the system you choose. Select a system that will not require vast training and support. Identify a manufacturer who offers support if you experience technical issues. Choose a brand that is of the best quality for accuracy and efficiency.